Afp Surveillance Stool Sample Collection

The annual proportion of afp cases with non polio enterovirus findings varied widely across the study period.

Afp surveillance stool sample collection.

Collection of two stool specimens 24 to 48 hours apart in the first 14 days following the onset of paralysis packed on ice fill in afp case investigation form include epid not usual nhls request form afp cif is packed with sample serves as request for the test send specimen to nicd 60 day follow up where indicated incompletely investigated cases classification of cases by npec. Highly sensitive surveillance for acute flaccid paralysis afp including immediate case investigation and specimen collection are critical for the detection of wild poliovirus circulation with the ultimate objective of polio eradication. The first specimen should be collected at the time of the case investigation. According to the who specified national targets for afp surveillance at least 80 of stool specimens must arrive at the laboratory within 72 hours of collection 35 37.

Surveillance is a cornerstone of polio eradication programs. The primary means of detecting poliovirus is through surveillance for acute flaccid paralysis afp among children aged 15 years with testing of stool specimens for wpv and vaccine derived polioviruses vdpvs in world health organization who accredited laboratories of the global polio laboratory network gpln 1 2. Results from this study reveal that this target remained out of reach throughout the study period. Our results indicated that the country performed well above the target throughout the study period.

Acute flaccid paralysis afp surveillance is the primary means of poliovirus detection supplemented by environmental surveillance i e the collection of sewage samples for poliovirus testing to identify poliovirus circulation in the absence of detected afp cases. Who has specified timeliness of transportation of stool specimens for afp surveillance at least 80 of stool specimens to arrive at the polio laboratory within 72 hours of sending from the field. Surveillance is one of the main pillars of the polio eradication initiative. In case samples cannot be collected within 14 days the specimens should still be collected up to 60 days of paralysis onset.

In order to maximise the ability of surveillance to rapidly detect imported cases the established performance indicators for afp surveillance requires that all cases of afp be notified and investigated as prospective polio cases including the collection of 2 stool samples 24 hours apart and within 14 days of the onset of paralysis 8 9. Stool specimens must be collected within 14 days of onset of paralysis to maximize the chances of isolating poliovirus. 2 stool specimens must be collected from every afp case.